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用β－肌动蛋白（β－ａｃｔｉｎ）作内参照，设计不同扩增片段的谷胱苷肽－Ｓ转移酶（ＧＳＴ－π）和多药耐药基因（ＭＤＲ１）引物，建立同时定量检测ＧＳＴ－π和ＭＤＲ１ｍＲＮＡ表达水平的复合定量ＰＣＲ法。扩增条带光密度值用凝胶成像系统检测，基因相对表达水平用目的基因和β－ａｃｔｉｎ光密度比值计算。本法灵敏度高、重复性好，能作批量分析。室内Ｘ控制图可有效控制定量ＰＣＲ扩增和电泳质量，保证本法能检测出小于２倍的表达差异。在乳腺癌组织中ＧＳＴ－π和ＭＤＲ１ｍＲＮＡ中位数分别为０．２７和１．３２，且表达水平呈明显正相关（ｒ＝０．３２３，Ｐ＜０．０５）。
GST-π and MDR1 primers were designed by using β-actin as an internal reference, and simultaneous detection of GST -π and MDR1 mRNA expression levels by multiplex quantitative PCR. The optical density of the amplified bands was detected by gel imaging system, and the relative gene expression level was calculated by the optical density ratio of the target gene and β-actin. The method of high sensitivity, good repeatability, can make batch analysis. Indoor X control chart can effectively control quantitative PCR amplification and electrophoresis quality, to ensure that this method can detect less than 2 times the expression difference. The median of GST-π and MDR1 mRNA in breast cancer tissues were 0.27 and 1.32, respectively, and the expression level was positively correlated (r = 0.323, P <0.05).