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建立了大鼠在体皮肤微透析技术,并应用于创伤涂膜剂经皮给药的药动学研究。6只SD大鼠在麻醉状态下进行皮肤微透析预处理后,将创伤涂膜剂涂于探针所在的皮肤表面,进行皮肤微透析。使用LC-MS/MS法测定透析液样品及大鼠血浆中的替硝唑、达克罗宁和氯己定。创伤涂膜剂经皮给药后替硝唑、达克罗宁和氯己定在大鼠皮肤和血浆中的tmax分别为(1.17±0.05)、(1.28±0.39)、(1.28±0.39)h和(1.36±0.38)、(1.64±0.24)、(1.43±0.35)h,cmax分别为(363.24±276.03)、(419.33±184.85)、(15.26±6.51)ng/ml和(96.27±43.72)、(20.12±12.98)、(9.86±5.07)ng/ml。
A rat dermal microdialysis technique was established and applied to the pharmacokinetic study of transdermal drug delivery. Six SD rats were subjected to skin microdialysis pretreatment under anesthesia. The wound coating agent was applied to the surface of the skin where the probe was located to perform skin microdialysis. Dialysate samples and rat plasma tinidazole, dacronin and chlorhexidine were determined by LC-MS / MS. The tmax values of tinidazole, dacronin and chlorhexidine in rat skin and plasma after transdermal wound dressing were (1.17 ± 0.05), (1.28 ± 0.39), (1.28 ± 0.39) h And (1.36 ± 0.38), (1.64 ± 0.24) and (1.43 ± 0.35) h respectively, and the values of cmax were (363.24 ± 276.03), (419.33 ± 184.85), (15.26 ± 6.51) ng / ml and (96.27 ± 43.72) (20.12 ± 12.98), (9.86 ± 5.07) ng / ml.