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壶腹嵴在快速旋转运动中是否会受损伤,检测毛细胞活力有助于其判断。用豚鼠39只,8只作对照,11只做脉冲噪声损伤实验,另20只分成4组,每组5只,做旋转损伤实验,其中3组分别以500r/min、1000r/min、1400r/min,每次旋转1min,等停稳后再重复实验,如此20次。而其余5只做1400r/min的1次旋转。全部完成后立即断头处死,取出壶腹嵴,分离毛细胞,并用1%台盼蓝做毛细胞染色,观察计数分析对毛细胞活力的影响。结果表明,多次快速旋转实验组和脉冲噪声损伤组的蓝染毛细胞数及其比例明显高于对照组,与噪声损伤组相似,并且蓝染毛细胞Ⅰ型和Ⅱ型均有明显升高,Ⅰ型毛细胞升高更显著。推测毛细胞活力下降。提示,多次快速旋转运动,有可能会引起内耳壶腹嵴感觉上皮毛细胞活力损伤。一次性快速短时间旋转未立即出现明显的细胞活力损伤。快速多次和噪声损伤与长时期缓慢旋转运动损伤之间表现出一致性。
Ampulla ridges in the rapid rotation of the movement will be damaged, detecting hair cell activity contribute to its judgment. 39 guinea pigs and 8 rabbits were used as controls. Eleven rabbits were randomly divided into four groups (n = 5). The rats in each group were exposed to 500r / min, 1000r / min and 1400r / min, each rotation 1min, wait for the stability and then repeat the experiment, so 20 times. While the remaining 5 do a rotation of 1400r / min. Immediately after the completion of all the decapitated sacrifice, remove the ampulla, separation of hair cells, and 1% trypan blue staining of hair cells to observe the counting analysis of the impact on hair cell viability. The results showed that the number and ratio of blue staining hair cells in the groups of rapid rotation and impulsive noise were significantly higher than those in the control group, which were similar to those in the noise injury group, and the type Ⅰ and type Ⅱ of blue staining hair cells were significantly increased , Type Ⅰ hair cells increased more significantly. Speculated that hair cell viability decreased. Tip, repeatedly rapid rotation, may cause the inner ear ampulla femoral epidermal epithelium sensory injury. One-time rapid short-term rotation did not immediately apparent significant cell viability damage. Consistency between rapid multiple and noisy injuries and long-term slow rotational motor injury was demonstrated.