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目的探讨缺氧诱导因子1α(HIF1α)在胃癌组织中的表达及其临床意义。方法采用免疫组化SP法,检测74例经手术切除的人体胃癌组织(术前均未行放疗及化疗)中HIF1α的表达。结果HIF1α主要表达于胃癌细胞浆及胞核,肿瘤间质细胞也可见少量表达,肿瘤坏死区周围及肿瘤浸润边缘部的癌细胞HIF1α表达明显增多。HIF1α的表达强度与细胞分化程度(P<0.05)、胃癌淋巴结转移(P<0.05)及预后(P<0.01)有关,而与病人年龄、性别及原发肿瘤大小均无关。其阳性强度随癌细胞分化程度的降低、淋巴结转移、术后生存期短而增高。结论HIF1α可作为评估胃癌淋巴结转移和预后的重要指标。
Objective To investigate the expression and clinical significance of hypoxia-inducible factor-1α (HIF1α) in gastric cancer. Methods Immunohistochemical SP method was used to detect the expression of HIF1α in 74 cases of human gastric cancer tissues that were surgically removed (no radiotherapy and chemotherapy before surgery). Results HIF1α was mainly expressed in the cytoplasm and nucleus of gastric cancer cells. Small interstitial cells were also found in tumor interstitial cells. The expression of HIF1α in the tumor necrosis area and the tumor infiltrating edge was significantly increased. The intensity of HIF1α expression was related to the degree of cell differentiation (P<0.05), lymph node metastasis (P<0.05), and prognosis (P<0.01), but not to the age, gender, and primary tumor size. The positive intensity increases with the degree of differentiation of cancer cells, lymph node metastasis, and short-term survival. Conclusion HIF1α can be used as an important index to evaluate the lymph node metastasis and prognosis of gastric cancer.