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目的观察紫杉醇对血管外膜成纤维细胞(AF)增殖、表型转化和迁移的影响。方法选取雄性SD大鼠,用贴壁法培养胸主动脉外膜AF,用免疫荧光法检测AF增殖细胞核抗原(PCNA),用流式细胞仪检测细胞周期,用Transwell法测定细胞迁移能力。结果 10 nmol.L-1紫杉醇对成纤维细胞存活率较空白组无明显差异,表明其对成纤维细胞无明显杀伤作用。与空白组比较,9 nmol.L-1紫杉醇可显著增加G0/G1期AF细胞百分比(P<0.05),降低G2/M及S期细胞百分比(P<0.05),并显著降低AF细胞PCNA表达,抑制其迁移能力(P<0.01)。结论低浓度紫杉醇在不影响成纤维细胞生存活力的情况下,能显著抑制细胞增殖和迁移能力。
Objective To observe the effects of paclitaxel on the proliferation, phenotypic transformation and migration of vascular adventitial fibroblasts (AF). Methods Male Sprague-Dawley rats were used. Adventitial thoracic aortic adventitial was cultured with adherent method. AF proliferating cell nuclear antigen (PCNA) was detected by immunofluorescence. Cell cycle was measured by flow cytometry. Cell migration was measured by Transwell method. Results 10 nmol.L-1 paclitaxel on fibroblasts survival rate compared with the blank group no significant difference, indicating that the fibroblasts have no significant killing effect. Compared with the blank group, 9 nmol.L-1 paclitaxel significantly increased the percentage of AF cells in G0 / G1 phase (P <0.05), decreased the percentage of G2 / M and S phase cells (P <0.05), and significantly reduced the expression of PCNA in AF cells , Inhibit their migration ability (P <0.01). Conclusion Low concentration paclitaxel can significantly inhibit cell proliferation and migration without affecting the viability of fibroblasts.