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目的通过集合HCV-RNA RT-PCR的方法,对宁夏丙型肝炎年发病率估算。方法采用50∶1、10∶1、1∶13级集合HCV-RNA RT-PCR方法及用于Pooling估计丙型肝炎年发病率的公式进行发病率估计。结果收集血浆共1 210份,2次采用酶联免疫吸附法(ELISA)检测丙型肝炎病毒(HCV)抗体阳性170例,抗体阳性率为14.1%;集合检测HCV抗体阴性样本共计1 040例,最后检测到HCV-RNA阳性样本36例,丙型肝炎病毒(HCV)年发病率为5.1%~8.7%。结论采用Pooilng方法对高危人群发病率预估是行之有效的。
Objective To estimate the annual incidence of hepatitis C in Ningxia by collecting HCV-RNA RT-PCR. Methods The prevalence of 50%, 10:1, 1:13 HCV-RNA RT-PCR and the formula used to estimate annual incidence of hepatitis C in the pool were estimated. Results A total of 1 210 plasma samples were collected and 170 samples of HCV were detected by enzyme-linked immunosorbent assay (ELISA) twice. The antibody positive rate was 14.1%. A total of 1040 HCV antibody negative samples were collected, Finally, 36 HCV-RNA positive samples were detected. The annual incidence of hepatitis C virus (HCV) was 5.1% -8.7%. Conclusions The Pooilng method is effective in predicting the incidence of high-risk population.