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目的:体外观察塞来昔布对人鼻咽癌CNE-2细胞增殖和凋亡的影响。方法:采用MTT比色法观察其对鼻咽癌细胞生长的影响;透射电子显微镜检测细胞凋亡,应用流式细胞术观察DNA的含量和凋亡的变化情况,TUNEL染色法计数细胞凋亡指数。结果:体外塞来昔布抑制CNE-2细胞生长,呈浓度和时间依赖性。透射电镜观察到细胞皱缩、胞质丢失、核质浓缩以及凋亡小体形成等凋亡形态学改变。流式细胞术显示细胞凋亡率显著增高,在80、100μmol/L浓度下细胞凋亡率分别为(10.47±0.18)%和(20.17±0.55)%,与对照组(1.57±0.27)%比较差异均有统计学意义;塞来昔布使G0/G1期细胞比例升高,S和G2/M期比例下降,呈一定剂量效应关系。TUNEL染色法显示药物组凋亡率明显高于对照组(P<0.01)。结论:塞来昔布能抑制人鼻咽癌CNE-2细胞增殖,并诱导其凋亡;其机制可能与诱导凋亡和阻止细胞周期进展有关。
Objective: To observe the effect of celecoxib on the proliferation and apoptosis of human nasopharyngeal carcinoma cell line CNE-2 in vitro. Methods: MTT colorimetric method was used to observe the effect of NPC on the growth of nasopharyngeal carcinoma cells; apoptosis was detected by transmission electron microscopy; DNA content and apoptosis were observed by flow cytometry; apoptosis index . RESULTS: Celecoxib inhibited the growth of CNE-2 cells in vitro in a concentration- and time-dependent manner. Transmission electron microscopy showed cell shrinkage, loss of cytoplasm, condensation of nuclei and formation of apoptotic bodies such as apoptotic morphological changes. Flow cytometry showed that the apoptotic rate was significantly higher than that of the control group (10.47 ± 0.18)% and (20.17 ± 0.55)% respectively at the concentrations of 80 and 100 μmol / L, compared with the control group (1.57 ± 0.27)% The difference was statistically significant; Celecoxib G0 / G1 phase cells increased the proportion of S and G2 / M phase decreased, showing a dose-response relationship. TUNEL staining showed that the apoptosis rate of the drug group was significantly higher than that of the control group (P <0.01). CONCLUSION: Celecoxib can inhibit CNE-2 cell proliferation and induce its apoptosis. Its mechanism may be related to inducing apoptosis and preventing cell cycle progression.