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目的:通过观察藏红花酸对肝纤维化小鼠肝组织病理学改变及p38MAPK蛋白表达的影响,探讨其抗肝纤维化作用及可能的机制。方法:36只6周龄雄性昆明小鼠随机分为3组:藏红花酸组、模型组、正常组。除正常对照组外,其余两组小鼠给予30%四氯化碳橄榄油溶液腹腔注射,首次注射5 m L/Kg,以后每次3 m L/Kg,每3日1次,连续12周。同时藏红花酸组给予藏红花酸5mg/Kg灌胃,每日1次,连续12周。实验过程中,观察各组小鼠的一般状态。12周末处死小鼠,肝组织切片行HE、Masson染色,显微镜下观察病理改变,免疫组化检测小鼠肝组织p38MAPK蛋白的表达。结果:实验过程中,模型组小鼠一般状态较差,体重增长缓慢;藏红花酸组小鼠一般状态尚可,体重较模型组增长明显(P<0.05)。肝组织HE、Masson染色结果显示:模型组小鼠肝纤维化程度较重(造模成功),与其相比,藏红花酸组小鼠肝纤维化程度有所改善,差异有统计学意义。与模型组相比,藏红花酸组肝组织p38MAPK表达显著下降(P<0.05)。结论:藏红花酸能有效减轻小鼠肝损伤及纤肝维化程度,其抗肝纤维化的机制可能与下调p38MAPK蛋白的表达有关。
OBJECTIVE: To observe the effect of crocetin on the pathological changes of hepatic tissue and the expression of p38MAPK protein in mice with liver fibrosis and to explore its anti-hepatic fibrosis and its possible mechanism. Methods: Thirty - six male Kunming mice of 6 weeks old were randomly divided into three groups: crocetin, model group and normal group. Except normal control group, the other two groups were given intraperitoneal injection of 30% CCl 4 olive oil solution, first injection of 5 m L / Kg, then 3 m L / Kg each time, once every 3 days for 12 weeks . At the same time crocetin given saffron acid 5mg / Kg gavage once daily for 12 weeks. During the experiment, the general status of mice in each group was observed. Mice were sacrificed on the 12th week. The liver sections were stained with HE and Masson. The pathological changes were observed under a microscope. The expression of p38MAPK protein in the liver tissues was detected by immunohistochemistry. Results: During the experiment, mice in the model group were generally in poor condition with slow weight gain. The mice in the crocetin group were generally tolerated and their body weights increased significantly compared with the model group (P <0.05). The results of HE and Masson staining showed that the degree of hepatic fibrosis in model group was higher than that in model group (model making success). Compared with the model group, the degree of hepatic fibrosis in mice in the crocetin group was improved, the difference was statistically significant. Compared with the model group, the expression of p38MAPK in the cysteic acid group was significantly decreased (P <0.05). Conclusion: Crocetin can effectively reduce liver injury and fibrosis in mice. The mechanism of anti-hepatic fibrosis may be related to down-regulation of p38MAPK protein expression.