本文研究了三套不同来源的黑籽和黄籽甘蓝型油菜近等基因系种皮中的总酚、花色素、类黄酮、黑色素含量及其相关的多酚氧化酶、苯丙氨酸解氨酸、酪氨酸酶活性在种子发育过程中的动态变化。实验结果表明 ,种皮中总酚、花色素及类黄酮在种子发育的前期逐渐增加 ,在开花 35至 4 3天后 ,其含量开始下降 ,PPO和 PAL活力与总酚和花色素的变化趋势一致 ;黑色素呈单调上升趋势 ,尤其是在种子成熟时 ,它在黑籽种皮中的含量迅速增加 ,是同时期黄籽油菜的 6倍多 ;酪氨酸酶也与黑色素的变化趋势一致。方差分析表明 ,多酚、类黄酮、花色素和黑色素在黑籽与黄籽种皮中的差异达到了极显著水平。在初期和中期 ,黑籽与黄籽色素含量的差异主要是与多酚、类黄酮和花色素有关。而在种子发育的后期 ,黑籽和黄籽油菜种皮色泽的差异最终主要是由黑色素所决定的 In this paper, the total phenols, anthocyanidins, flavonoids, melanin contents and their related polyphenol oxidases in the seed coat of near isogenic lines of three different origins of black and yellow-seeded Brassica napus were studied. Phenylalanine ammonia- Dynamic Changes of Acid and Tyrosinase Activities during Seed Development. The results showed that the total phenols, anthocyanins and flavonoids in the seed coat increased gradually in the early stage of seed development. After 35 to 43 days of flowering, the contents of total phenols, anthocyanidins and flavonoids began to decline, and the changes of PPO and PAL activity were consistent with the changes of total phenolic and anthocyanidins ; Melanin monotonous upward trend, especially in the seed maturity, its content in the black seed coat rapidly increased six times more than the same period yellow rapeseed; tyrosinase also consistent with the trend of melanin. Analysis of variance showed that the differences between polyphenols, flavonoids, anthocyanidins and melanin in black seed and yellow seed coat reached extremely significant levels. In the early and mid-term, the differences in pigment content between black and yellow seeds were mainly related to polyphenols, flavonoids and anthocyanidins. In the later stages of seed development, however, the differences in seed coat color between black and yellow seed rape are ultimately dominated by melanin