作者发展了一种改进型的拉直DNA分子以供光学显微镜下观察限制酶切图谱的方法。DNA分子在覆盖有3-氨丙基三乙氧基甲硅烷的载玻片表面,通过移动的弯月面形成的表面张力而被拉直。在实验中,弯月面的运动被机械控制,运动速度在0.5cm/min至5cm/min之间。这有利于速度控制和被拉直的分子的均一性的形成。硅烷化表面对DNA分子的亲和力的差异由事先在载玻片表面覆盖单链 The authors developed an improved method of straightening DNA molecules for the observation of restriction maps by light microscopy. The DNA molecules were straightened on the surface of the slide covered with 3-aminopropyltriethoxysilane by the surface tension created by the moving meniscus. In experiments, the movement of the meniscus was mechanically controlled at a speed of between 0.5 cm / min and 5 cm / min. This facilitates the formation of velocity control and homogeneity of straightened molecules. Differences in affinities of the silanized surface to DNA molecules are preceded by a single strand covering the slide surface