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目的:采用高效液相色谱-直流安培检测法建立扶本增脉颗粒中丹酚酸B的含量测定方法。方法:采用ODS-BP C18柱(200mm×4.6mm,5μm);以乙腈和0.1%磷酸(35:65,v/v)为流动相(流速1.0mL/min)进行等度洗脱;工作电极为Pt,参比电极为Ag/AgCl,工作电压为1.0V;柱温为30℃。结果:丹酚酸B质量浓度分别为0.005-10mg/L时与其峰面积呈良好的线性关系(r2=0.9998),回收率为97.8%-100.4%;相对标准偏差(RSD)<5.0%,检测限为2μg/L。结论:该方法与高效液相色谱-紫外检测法相比,含量测定结果一致,检测限更低,是一种快速、灵敏、准确的分析方法,可以为扶本增脉颗粒的质量控制提供参考。
OBJECTIVE: To establish a method for the determination of salvianolic acid B in Fubenzaomai granules by high performance liquid chromatography-direct current amperometry. METHODS: ODS-BP C18 column (200mm × 4.6mm, 5μm) was used for isocratic elution with acetonitrile and 0.1% phosphoric acid (35:65, v / v) As Pt, the reference electrode is Ag / AgCl, the working voltage is 1.0V; the column temperature is 30 ℃. Results: The linear range of the concentration of salvianolic acid B was 0.005-10 mg / L (r2 = 0.9998), the recovery was 97.8% -100.4%, the relative standard deviation (RSD) was less than 5.0% Limit 2μg / L. Conclusion: Compared with HPLC - UV method, this method has the same determination results and lower detection limit, which is a fast, sensitive and accurate analytical method, which can provide reference for the quality control of Fubenzaomai granules.