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本文介紹利用短期体外培养吉田腹水肉瘤細胞进行药物篩选的方法.所用培养基为馬血清平衡盐溶液,每毫升接种細胞数为6—10万,于加入药物后24-48小时进行細胞計数,計算药物对細胞生长的抑制百分率,并以形态观察作为輔助指标. 曾进行5种已知抗肿瘤药的敏感試驗,发現药物的作用强度为:氮芥>氧化氮芥>新恩必恨>丁二醇二甲磺酸酯(myleran)>6-巯基嘌呤.此外,过篩了24种合成药和93种天然药物成分,其中有作用者分別为41.7％和38.7％.将篩选結果与动物瘤体內結果作比較,发現二者间有一定的相关.最后对方法的优缺点进行了討論.
This article describes the use of short-term in vitro culture of Yoshida ascites sarcoma cells for drug screening methods used horse serum equilibrated salt solution, the number of cells per ml of inoculation of 6-10 million, adding drugs 24-48 hours after the cell count, Calculate the percent inhibition of drug on cell growth, and morphological observation as an auxiliary index.We conducted five kinds of known antitumor drug sensitivity test and found that the strength of the drug action: nitrogen mustard> nitrogen oxide mustard> new grace must hate> Butylenesulfonate was more than 6-mercaptopurine.In addition, 24 synthetic drugs and 93 natural medicine components were screened, 41.7% and 38.7% of the active ingredients were screened respectively The results of animal tumor comparison, found that there is a certain correlation between the two.Finally, the advantages and disadvantages of the method were discussed.