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应用ＨＰＬＣ－ＥＣＤ法研究了大鼠脑缺血及复灌期间纹状体多巴胺（ＤＡ）的变化以及ＮＭＤＡ受体拮抗剂氯胺酮（Ｋｅｔａｍｉｎｅ，ＫＴ）对脑缺血纹状体内ＤＡ含量变化的影响。四动脉阻断致急性脑缺血１０ｍｉｎ及缺血１０ｍｉｎ复灌１０ｍｉｎ、３０ｍｉｎ和６０ｍｉｎ，均可见纹状体ＤＡ含量显著减少（Ｐ＜０．０１）。这可能与脑缺血及复灌早期脑组织ＤＡ释放增加和摄取减少有关。ＫＴ２５ｍｇ·ｋｇ－１和５０ｍｇ·ｋｇ－１均可拮抗脑缺血及复灌时ＮＭＤＡ受体介导的纹状体ＤＡ含量减少。
The changes of dopamine (DA) in the striatum during cerebral ischemia and reperfusion in rats and the effect of Ketamine (KT), an NMDA receptor antagonist, on the DA content in cerebral ischemic striatum were studied by HPLC-ECD. The DA content in the striatum was significantly decreased (P <0.01) in 10 minutes, 30 minutes and 60 minutes after 10 minutes, 10 minutes and 10 minutes after ischemia. This may be related to cerebral ischemia and reperfusion early brain tissue DA increased release and decreased intake. Both KT25mg · kg-1 and 50mg · kg-1 could antagonize the decrease of NMDA receptor-mediated striatal DA during cerebral ischemia and reperfusion.