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目的:实验结果表明4HPR抑制Hela细胞生长的机理之一是诱导该细胞凋亡。本文用流式细胞仪检测不同浓度的4HPR作用不同时间Hela细胞凋亡的百分比及细胞周期变化。方法:体外细胞培养及流式细胞仪检测法。结果:2μg/m的4HPR作用24、48、72h后,Hela细胞的凋亡百分比分别为4.1%、6.6%、26%;4μg/ml的4HPR分别为5.1%、8.3%、28%、8μg/ml的4HPR分别为5.9%、6.2%、34%。细胞周期分布图中,G1细胞明显减少。结论:上述结果提示Hela细胞凋亡的百分比与4HPR的作用时间、作用浓度呈正相关。
OBJECTIVE: The experimental results show that one of the mechanisms of 4HPR inhibiting the growth of Hela cells is to induce apoptosis of the cells. In this study, flow cytometry was used to detect the percentage of Hela cells apoptosis and cell cycle changes at different concentrations of 4HPR. Methods: In vitro cell culture and flow cytometry assays. RESULTS: After 24 h, 48 h, and 72 h of 2μg/m 4HPR treatment, the apoptosis percentage of Hela cells was 4.1%, 6.6% and 26%, respectively; the 4HPR of 4 μg/ml was 5.1% and 8.3%, respectively. The 4HPRs of 28% and 8 μg/ml were 5.9%, 6.2%, and 34%, respectively. G1 cells were significantly reduced in the cell cycle distribution. Conclusion: These results suggest that the percentage of apoptosis in Hela cells is positively correlated with the duration of action and concentration of 4HPR.