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目的比较不同剂量(接近临床常用剂量)的海藻和甘草对甲状腺肿大大鼠的治疗作用,并探讨海藻的不同品种对治疗作用机制的影响。方法大鼠连续灌服丙硫氧嘧啶14天复制甲状腺肿大模型,并将其分为空白组、模型组、阳性对照组、海藻(海蒿子)玉壶汤高剂量组、海藻(海蒿子)玉壶汤中剂量组、海藻(海蒿子)玉壶汤低剂量组、海藻(羊栖菜)玉壶汤高剂量组、海藻(羊栖菜)玉壶汤中剂量组、海藻(羊栖菜)玉壶汤低剂量组,共9组。除空白组外,其余各组大鼠每日灌服丙硫氧嘧啶1次,质量浓度为1 mg/(mL·100 g),连续灌服14天。14天后,空白组和模型组每天灌服等量的生理盐水,其余各组灌服相应药物,体积均为1 mL/100 g,连续给药28天,期间为了稳定模型,每隔1天灌服丙硫氧嘧啶1次,剂量与之前一致。给药结束后,计算各组大鼠甲状腺系数,并测定甲状腺激素三碘甲状腺原氨酸(three iodine thyroid 3,5,3’-triiodothyronine,T_3)、四碘甲状腺原氨酸(thyroxine,T_4)、游离三碘甲状腺原氨酸(free triiodothyronine,FT_3)、游离四碘甲状腺原氨酸(free thyroxine,FT_4)、促甲状腺激素(thyroid-stimulating hormone,TSH)、促甲状腺激素释放激素(thyrotropin-releasing hormone,TRH)水平。结果 (1)各给药组T_3水平较模型组不同程度升高,其中羊高组略优于海高组,且均分别优于各中、低剂量组(P<0.05或P<0.01);(2)各给药组在FT_3水平上均体现出向正常转归的趋势,其中海高组优于阳性药组及其他各组(P<0.05或P<0.01);(3)与模型组相比,海低组及羊栖菜各组的TSH水平均有显著的下降趋势(P<0.01),且效果均优于阳性药组,其中羊高组效果最佳。结论含不同品种海藻的海藻玉壶汤对由甲状腺肿大引起的各种甲状腺激素水平的变化均有一定程度的恢复作用,其中以海藻玉壶汤(海蒿子)高剂量组、海藻玉壶汤(羊栖菜)高剂量组效果为佳。
Objective To compare the therapeutic effects of seaweed and licorice at different doses (close to the commonly used clinical dose) on goiter in rats and explore the effects of different species of seaweed on the therapeutic mechanism. Methods The rats were randomly divided into three groups: control group, model group, positive control group, high dose of seaweed (sea cucumber) Yuhu Tang group, seaweed (sea cucumber) The middle-dose group of Yuhu Tang, the low-dose group of seaweed (Artemisia selengensis) and Yuhu Tang, the high-dose group of seaweed (Prunus ceragee) and Yuhu Tang, the middle dose of seaweed ) Yuhu Tang low-dose group, a total of 9 groups. Except the blank group, the other rats in each group were given propylthiouracil once daily for 1 day at a concentration of 1 mg / (mL · 100 g) for 14 days. After 14 days, the rats in the blank group and the model group were given the same amount of saline daily, while the other groups were given the corresponding drugs in a volume of 1 mL / 100 g for 28 consecutive days. In order to stabilize the model, Serum propylthiouracil 1, the same dose as before. After administration, the thyroid coefficient of each group was calculated and the levels of thyroid hormone, thyroid hormone (T_3), thyroxine (T_4) , Free triiodothyronine (FT_3), free thyroxine (FT_4), thyroid-stimulating hormone (TSH), thyrotropin-releasing hormone (thyrotropin-releasing hormone) hormone, TRH) levels. Results (1) Compared with the model group, the T_3 level of each administration group increased to some extent, and the sheep high group was slightly better than the sea high group, which were better than the middle and low dose groups (P <0.05 or P <0.01); 2) The FT_3 level of each administration group showed the trend of normal return, of which the sea high group is superior to the positive drug group and other groups (P <0.05 or P <0.01); (3) Compared with the model group (P <0.01). The results showed that the TSH levels in all groups were significantly decreased (P <0.01), and the effect was better than the positive drug group, of which sheep group was the best. Conclusion The seaweed juguang decoction with different species of seaweed had a certain degree of recovery on the changes of thyroid hormones caused by goiter. Among them, the seaweed juguang soup (Habitat Sargassum) high dose group effect is better.