Maneckfee和Minna应用特异的放射性配基受体亲合实验,发现多种肺癌细胞株均可表达高亲合性的μ、δ、κ型阿片受体,也能表达烟碱和α-银环蛇毒受体。培养液内加入阿片肽和烟碱后,肺癌细胞的cAMP含量降低,说明这些受体是具有生物活性的。应用放射免疫分析法发现,肺癌细胞还能表达多种具有免疫活性的阿片肽,如β-内啡肽、脑啡肽及强啡肽。在培养体系内加入1～100 nmol/L的μ、δ、κ阿片受体激 Using specific radioligand receptor affinity experiments, Maneckfee and Minna found that many lung cancer cell lines express high-affinity μ, δ, κ opioid receptors as well as nicotine and α-bungarotoxin Receptor. The addition of opioid peptides and nicotine to the culture fluid resulted in a decrease in cAMP levels in lung cancer cells, indicating that these receptors are biologically active. Application of radioimmunoassay found that lung cancer cells can express a variety of immunocompetent opioids, such as β-endorphin, enkephalin and dynorphin. In the culture system by adding 1 ~ 100 nmol / L of μ, δ, κ opioid receptor stimuli