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用Ｐ１增强的３２Ｐ－后标记方法测定了经腹腔注射染苯小鼠不同组织ＤＮＡ加合物的分布及其与苯引起的外周血白细胞（ＷＢＣ）减少、淋巴细胞微核形成及骨髓细胞染色体畸变的关系。结果表明：１．苯在小鼠骨髓、肝脏和外周血ＷＢＣ均可形成ＤＮＡ加合物，其加合物含量以骨髓最高，肝脏次之，ＷＢＣ最低。本结果与苯的代谢及毒性作用特点相一致。２．ＤＮＡ加合物形成与细胞毒性有关系，ＤＮＡ加合物量增加，淋巴细胞微核及骨髓细胞染色体畸变亦增加，而外周血白细胞数明显减少，显示细胞遗传毒性和细胞毒性均增大。本研究为ＤＮＡ加合物作为生物学监测指标，筛选高危人群提供了有力的实验依据。
The distribution of DNA adducts in different tissues of mice with intraperitoneal injection of benzene and the decrease of benzene-induced peripheral blood leukocytes (WBC), micronuclei of lymphocytes and chromosomal aberrations of bone marrow cells were determined by P1P-enhanced 32P- Relationship. The result shows: 1. Benzene in mice bone marrow, liver and peripheral blood WBC can form DNA adducts, adduct content to the highest bone marrow, followed by the liver, WBC lowest. This result is consistent with the metabolic and toxic effects of benzene. 2. The formation of DNA adducts was related to cytotoxicity. The amount of DNA adducts increased, the chromosome aberrations of lymphocytes micronucleus and bone marrow cells increased, while the number of leukocytes in peripheral blood decreased significantly, which indicated that both cytoxicity and cytotoxicity increased. In this study, DNA adducts as a biological monitoring indicators, screening of high-risk groups provide a strong experimental basis.