论文部分内容阅读
目的:观察雷公藤提取物长期用药对大鼠心脏毒性的影响。方法:将SD大鼠随机分为空白对照组和雷公藤提取物低、中、高剂量组17,34,68 mg·kg-1,每组60只。各组ig给药1次/d,连续90 d,分别于给药前(0 d)及给药后15,30,60,90 d测定各组血钾、心电图及心功能指标,并采用HE染色法观察大鼠心肌组织的病理变化。结果:低剂量组与给药前或空白对照组比较血钾、心电图及心功能各指标无明显变化。中剂量组给药后60～90 d出现血钾降低,心电图指标如Q-T间期、S-T间期、QRS间期延长、心率降低和ST段偏移,心功能指标如左室收缩压(LVSP)降低、左室舒张末压(LVEDP)升高、左室内压最大上升/下降速度(±dp/dtmax)降低(P<0.05)。高剂量组在给药后15～30 d上述指标已有明显变化(P<0.05)。HE染色显示,给药后90 d低剂量组大鼠心肌未明显见病理变化,中、高剂量组大鼠心肌有明显损伤。结论:雷公藤提取物连续ig给药90 d后,17 mg·kg-1剂量组对大鼠心脏无明显毒性作用,34,68 mg·kg-1剂量组对大鼠心脏有毒性,且高剂量组毒性作用更为明显。
Objective: To observe the long-term administration of Tripterygium wilfordii extract on rat heart toxicity. Methods: SD rats were randomly divided into blank control group and tripterygium wilfordii extract low, medium and high dose group 17,34,68 mg · kg-1, 60 rats in each group. Each group ig administration 1 times / d, continuous 90 d, respectively, before administration (0 d) and after administration of 15, 30, 60, 90 d determination of serum potassium, ECG and cardiac function indicators, and using HE The pathological changes of myocardial tissue were observed by staining. Results: There was no significant change in serum potassium, ECG and cardiac function between low-dose group and pre-administration or blank control group. Serum potassium was decreased 60 to 90 days after administration in middle-dose group. ECG indexes such as QT interval, ST interval, QRS interval prolongation, heart rate reduction and ST segment deviation, cardiac function indexes such as left ventricular systolic pressure (LVSP) LVEDP and ± dp / dtmax decreased significantly (P <0.05). The high-dose group 15 ~ 30 d after administration of the above indicators have been significantly changed (P <0.05). HE staining showed that the pathological changes were not observed in the myocardium of the low-dose group for 90 days and the myocardial injury in the medium-dose and high-dose groups was obvious. CONCLUSION: Tripterygium wilfordii extract extract has no toxic effect on rat heart after 90 d continuous ig administration, and the dose of 34 and 68 mg · kg-1 is toxic to rat heart Toxicity dose group more obvious.