AIM: To screen lymph nodes metastasis associated long noncoding RNAs(lncRNAs) in colorectal cancer through microarray analysis.METHODS: Metastatic lymph node(MLN), normal lymph node(NLN) and tumor tissues of 3 colorectal cancer(CRC) patients were collected during the operation and validated by pathological examinations. RNAs were extracted from MLN, NLN, and cancer tissues separately. RNA quantity and quality were measured with a NanoDrop ND-1000 spectrophotometer and RNA integrity was assessed by standard denaturing agarose electrophoresis. Agilent Feature Extraction Software(Version 11.0.1.1) was used to analyze acquired array images. Four differently expressed lncRNAs were con-firmed by quantitative real-time polymerase chain reaction(qRT-PCR) in 26 subsets of MLN, NLN, and tumor tissues.RESULTS: Of 33045 lncRNAs, 1133 were differentially expressed in MLN compared with NLN, of which 260 were up-regulated and 873 down-regulated(≥ 2 foldchange). Five hundred and forty-five lncRNAs were differentially expressed in MLN compared with tumor tissues, of which 460 were up-regulated and 85 downregulated(≥ 2 fold-change). Compared with NLN and cancer tissues, 14 lncRNAs were specifically upregulated and 5 specifically down-regulated in MLN. AK307796, ENST00000425785, and AK021444 were confirmed to be specifically up-regulated in MLN and ENST00000465846 specifically down-regulated in MLN by qRT-PCR in 26 CRC patients.CONCLUSION: The specifically expressed lncRNAs in MLN may exert a partial or key role in the progress of lymph nodes metastasis of CRC. AIM: To screen lymph nodes metastasis associated long noncoding RNAs (lncRNAs) in colorectal cancer by microarray analysis. METHODS: Metastatic lymph node (MLN), normal lymph node (NLN) and tumor tissues of 3 colorectal cancer (CRC) patients were collected during the operation and validated by pathological examinations. RNA quantity and quality were measured with a NanoDrop ND-1000 spectrophotometer and RNA integrity was assessed by standard denaturing agarose electrophoresis. Agilent Feature Extraction Software ( Four different expressed lncRNAs were con-firmed by quantitative real-time polymerase chain reaction (qRT-PCR) in 26 subsets of MLN, NLN, and tumor tissues .RESULTS: Of 33045 Of the 260 were up-regulated and 873 down-regulated (≥ 2 fold change). Five hundred and forty-five lncRNAs w Compared with NLN and cancer tissues, 14 lncRNAs were specifically upregulated and 5 specifically down-regulated in MLN. AK307796 , ENST00000425785, and AK021444 were confirmed to be specifically up-regulated in MLN and ENST00000465846 specifically down-regulated in MLN by qRT-PCR in 26 CRC patients. CONCLUSION: The specifically expressed lncRNAs in MLN may exert a partial or key role in the progress of lymph node metastasis of CRC.