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目的探讨PCNA和Caspase-3在胃癌及癌前病变中的表达及其与Hp感染的关系。方法应用快速尿素酶试验与吉姆斯染色法将各种病变分为Hp感染阳性组和阴性组,应用免疫组化法对各种病变胃组织PCNA和Caspase-3的表达进行检测。结果①胃癌及癌前病变组PCNA表达及Hp感染率明显高于慢性浅表性胃炎组(P<0.05),而Caspase-3的表达显著低于慢性浅表性胃炎组(P<0.05);②Hp感染阳性组PCNA及Caspase-3表达明显高于Hp感染阴性组(P<0.05)。结论Hp感染可能通过促进PCNA及Caspase-3基因蛋白的过度表达,从而使胃黏膜细胞增生与凋亡平衡失调诱发癌变。
Objective To investigate the expression of PCNA and Caspase-3 in gastric carcinoma and precancerous lesions and their relationship with Hp infection. Methods Rapid urease test and Jams staining were used to classify various pathological changes into Hp positive and negative groups. The expressions of PCNA and Caspase-3 in gastric mucosa were detected by immunohistochemistry. Results ① The expressions of PCNA and Hp in gastric cancer and precancerous lesions were significantly higher than those in chronic superficial gastritis group (P <0.05), while the expression of Caspase - 3 was significantly lower than that in chronic superficial gastritis group (P <0.05). ② The expression of PCNA and Caspase-3 in Hp positive group was significantly higher than that in Hp negative group (P <0.05). Conclusion Hp infection may promote carcinogenesis by imbalanced proliferation of PCNA and Caspase-3 gene protein, which may lead to imbalance of proliferation and apoptosis in gastric mucosa.