论文部分内容阅读
目的探讨PCB153和PBDE-47单独及联合染毒对SH-SY5Y细胞氧化应激及8-羟基脱氧鸟苷(8-OHdG)含量的影响。方法设DMSO溶剂对照组,1、5、10μmol/LPBDE-47(低、中、高)单独染毒组和与5μmol/LPCB153联合染毒组及相应的抗氧化剂组(N-乙酰-L-半胱氨酸NAC100μmol/L),分别对SH-SY5Y细胞染毒24h。用荧光染料DCFH-DA标记法和高效液相色谱-电化学技术(HPLC-ECD)分别检测细胞内活性氧(ROS)水平和8-OHdG的含量。结果随着染毒剂量的增加,PBDE-47单独染毒组ROS水平有逐渐增高的趋势,但与对照组及其相应的PCB153联合组相比,ROS水平无明显差异(P>0.05)。中、高联合染毒组ROS水平与对照组相比显著增加(P<0.05),并显著高于相应的单独剂量染毒组(P<0.05),加入抗氧化剂后细胞内ROS水平明显下降(P<0.05),细胞内ROS水平与PBDE-47染毒剂量呈现剂量-效应关系;高剂量PBDE-47单独染毒组和中、高剂量联合染毒组8-OHdG的含量与对照组相比均有明显的增加(P<0.05),高剂量联合染毒组8-OHdG含量明显高于相应的单独染毒组(P<0.05),加入抗氧化剂后其细胞内8-OHdG含量明显下降(P<0.05)。细胞内ROS水平与8-OHdG含量呈正相关关系(r=0.895)。结论一定剂量的PBDE-47可致DNA氧化损伤,PCB153可增加PBDE-47对SH-SY5Y细胞DNA的氧化损伤作用,提示活性氧在PBDE-47致DNA损伤方面发挥了重要作用。
Objective To investigate the effects of PCB153 and PBDE-47 on oxidative stress and 8-hydroxydeoxyguanosine (8-OHdG) in SH-SY5Y cells. Methods DMSO solvent control group, 1,5,10μmol / LPBDE-47 (low, medium and high) single exposure group and 5μmol / L PCB153 combined exposure group and the corresponding antioxidant group (N-acetyl L- Cystine NAC100μmol / L), SH-SY5Y cells were exposed to 24h. The level of reactive oxygen species (ROS) and the content of 8-OHdG in cells were detected by fluorescent dye DCFH-DA labeling method and high performance liquid chromatography-electrochemistry (HPLC-ECD). Results With the increase of exposure dose, the level of ROS in PBDE-47 treated group tended to increase gradually, but there was no significant difference (P> 0.05) between the control group and its corresponding PCB153 combination group. Compared with the control group, ROS levels in the medium and high combined exposure groups were significantly increased (P <0.05), and were significantly higher than those in the corresponding single exposure groups (P <0.05). ROS levels were significantly decreased after the addition of antioxidants P <0.05). The level of intracellular ROS was dose-dependently correlated with the dose of PBDE-47. The content of 8-OHdG in high-dose PBDE-47 group and medium-dose and high-dose group were significantly higher than those in control group (P <0.05). The content of 8-OHdG in the high-dose combination group was significantly higher than that in the corresponding single exposure group (P <0.05), and the content of 8-OHdG in the combination group was significantly decreased P <0.05). There was a positive correlation between intracellular ROS level and 8-OHdG content (r = 0.895). Conclusion PBDE-47 can induce DNA oxidative damage. PCB153 can increase the oxidative DNA damage of SH-SY5Y cells induced by PBDE-47, suggesting that ROS plays an important role in the DNA damage induced by PBDE-47.