Imaging Escherichia coli using streptavidin functionalized quantum dots as a Nano-fluorescent probe

来源 :Journal of Harbin Institute of Technology | 被引量 : 0次 | 上传用户:QQ403402618
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It is extremely important for bacteria detection in many fields,such as medical diagnosis and food safety.In this paper,streptavidin functionalized quantum dots(SA-QDs),as a nano-fluorescent probe,were used to attach with Escherichia coli(E.coli) for the detection and identification of bacteria with immunoreactions and biotin-streptavidin affinity.Fluorescent images of the bacteria and the fluorescence intensity were used to evaluate the conjugation effect with different incubation time.Our results showed that 20 min is a reasonable incubation time for the SA-QDs coupling to E.coli cells.The fluorescent images,which produce a greatly amplified and enhanced signal of E.coli cells,were obtained through the immunological amplification and fluorescent probe enrichment steps.In addition,the bleaching process of SA-QDs without any encapsulation at room temperature was clearly observed during 10 min of being excited.Our work provided a modularized sample treatment method using SA-QDs as a nano-fluorescent probe in cellular imaging and bio-labeling. It is extremely important for bacteria detection in many fields, such as medical diagnosis and food safety. In this paper, streptavidin functionalized quantum dots (SA-QDs), as a nano-fluorescent probe, were used to attach with Escherichia coli (E. For the detection and identification of bacteria with immunoreactions and biotin-streptavidin affinity. Fluorescent images of the bacteria and the fluorescence intensity were used to evaluate the conjugation effect with different incubation time. Our results showed that 20 min is a reasonable incubation time for the SA-QDs coupling to E. coli cells. These fluorescent images, which produce a greatly amplified and enhanced signal of E. coli cells, were obtained through the immunological amplification and fluorescent probe enrichment steps. In addition, the bleaching process of SA- QDs without any encapsulation at room temperature was clearly observed during 10 min of being excited. Our work provided a modularized sample treatment method using SA-QDs as a nan o-fluorescent probe in cellular imaging and bio-labeling.
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