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目的:探讨天然牛磺酸是否具有排铅解毒的作用。方法:①实验于2004-10/12在广西区疾病预防控制中心毒理科完成。选用SPF级Wistar纯种雄性大白鼠55只。将检疫合格大鼠按体质量分层随机分为5组:牛磺酸高、中、低3个剂量组(以纯牛磺酸计),空白对照组,模型对照组,每组11只。②实验开始后,模型对照组、受试样品各剂量组每天饮用1g/L醋酸铅水溶液造模的同时,牛磺酸高、中、低剂量组动物灌胃给予纯牛磺酸0.07,0.14,0.21mg/kg,1次/d,连续30d;空白对照组不造成高铅模型,灌胃蒸馏水,灌胃量为10mL/kg,1次/d,连续30d。于末次给予受试药品24h后。采用瞬间高压直流电550V将大鼠电昏后处死,取血,取肝脏和股骨称重后进行湿式消化,用石墨炉原子吸收分光光度计测定铅含量。③计量资料差异比较采用方差分析。结果:大白鼠55只均进入结果分析。①血铅水平:牛磺酸高、中、低剂量组均明显低于模型对照组犤(2.49±0.31),(2.54±0.27),(2.40±0.29),(3.45±0.88)μg/L,P<0.01犦;模型对照组明显高于空白对照组犤(0.38±0.18)μg/L,P<0.01犦。②肝组织铅含量:牛磺酸高、中、低剂量组均明显低于模型对照组犤(1.73±0.64),(1.51±0.16),(1.45±0.63),(2.40±0.43)μg/g,P<0.01犦;模型对照组明显高于空白对照组犤(0.45±0.10)μg/g,P<0.01犦。股骨组织铅含量:牛磺酸高、中剂量组均明显低于模型对照组犤(3.86±1.20),(3.54±0.88),(5.00±1.34)μg/g,P<0.01犦;模型对照组明显高于空白对照组犤(0.12±0.05)μg/g,P<0.01犦。结论:天然牛磺酸具有促进血铅排出,降低肝组织、股骨内铅含量的作用。
Objective: To investigate whether natural taurine has the role of lead detoxification. Methods: ① The experiment was performed in the Department of Toxicology of Guangxi Center for Disease Control and Prevention from October to October 2004. 55 SPF Wistar male rats were used. Quarantine qualified rats were randomly divided into five groups according to their body mass distribution: high, medium and low doses of taurine (pure taurine), blank control group, model control group, 11 rats in each group. ② After the experiment began, the model control group and the test sample were dosed daily with 1g / L lead acetate aqueous solution while the taurine high, medium and low dose groups were given gavage with pure taurine 0.07,0.14, 0.21mg / kg once a day for 30 days. The blank control group did not induce high-lead model, and intragastric administration of distilled water was given. The intragastric administration was 10 mL / kg once daily for 30 days. After the last test drug to be given 24h. The rat was sacrificed after fainting by instant high-voltage DC 550V, blood was taken, and the liver and femur were weighed and wet-digested. The content of lead was determined by graphite furnace atomic absorption spectrophotometer. ③ measurement data were compared using variance analysis. Results: 55 rats were involved in the result analysis. ① Blood lead levels: The taurine levels in high, middle and low dose groups were significantly lower than those in model control group (2.49 ± 0.31), (2.54 ± 0.27), (2.40 ± 0.29) and (3.45 ± 0.88) μg / L, P <0.01 犦; The model control group was significantly higher than the blank control group 0. (0.38 ± 0.18) μg / L, P <0.01 犦. (2) Lead content in liver tissue: The taurine concentration in high, medium and low dose groups were significantly lower than that in model control group (1.73 ± 0.64, 1.51 ± 0.16, 1.45 ± 0.63, 2.40 ± 0.43μg / g , P <0.01 犦. The model control group was significantly higher than that of the blank control group (0.45 ± 0.10) μg / g, P <0.01 犦. The lead content in the femur tissue was significantly lower than that in the model control group (3.86 ± 1.20), (3.54 ± 0.88), (5.00 ± 1.34) μg / g, P <0.01 犦, respectively. The model control group Significantly higher than the blank control group 犤 (0.12 ± 0.05) μg / g, P <0.01 犦. Conclusion: Natural taurine has the effect of promoting blood lead excretion and reducing the content of lead in the liver tissue and femur.