论文部分内容阅读
目的:建立HPLC测定大鼠口服养阴通脑颗粒有效部位组合后血浆中葛根素浓度的方法。方法:以乙腈提取处理血浆,以对羟基苯甲酸为内标,甲醇-0·5%乙酸水(28:72,v:v)为流动相,ODSC18色谱柱(150mm×4·6mm,5μm)为固定相,流速为1·0mL/min,以紫外检测器在248nm波长下测定血浆中葛根素的吸收。结果:葛根素在0·025~3·2μg/mL范围内线性关系良好,RSD<10%,平均回收率为97·6%,最低检测限为0·16ng,血浆中最低检测浓度为0·016μg/mL。结论:此法灵敏、可靠,可较好地用于口服中药复方养阴通脑颗粒主要有效部位组合后血浆中葛根素的测定,可为中药复方体内成分分析提供方法。
Objective: To establish a HPLC method for the determination of puerarin in plasma after oral administration of Yangyintongnao granules. METHODS: The plasma was extracted with acetonitrile with p-hydroxybenzoic acid as the internal standard, and the methanol-0.5% acetic acid water (28:72, v:v) was used as the mobile phase. The ODSC18 column (150mm×4.6mm, 5μm) was used as the mobile phase. For the stationary phase, the flow rate was 1.0 mL/min, and the absorption of puerarin in plasma was measured with a UV detector at a wavelength of 248 nm. Results: The puerarin had good linearity in the range of 0·025~3·2μg/mL, RSD<10%, the average recovery was 97.6%, the detection limit was 0·16ng, the lowest detection concentration in plasma was 0· 016 μg/mL. Conclusion: This method is sensitive and reliable, and it can be used to determine the plasma puerarin after oral administration of traditional Chinese medicine compound Yangyintongnao granules. It can provide a method for the analysis of Chinese herbal compound in vivo.