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目的探讨人白细胞介素-4(IL-4)启动子-590C→T多态性与特发性血小板减少性紫癜(ITP)发病的可能关系。方法酶联免疫法测定血小板相关抗体IgG及IL-4水平,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)的方法分析94名ITP患者和106名正常健康志愿者IL-4启动子-590C→T的基因型和等位基因频率。结果ITP组血小板相关抗体IgG[(273.71±90.45)ng/107PA]明显高于正常组[(72.35±23.32)ng/107PA],同时血清IL-4水平[(92.43±20.69)ng/L]也明显高于正常组[(46.71±11.9)ng/L];在ITP组,IL-4启动子区-590位点C等位基因频率为0.16,T等位基因频率为0.84;正常对照组C等位基因频率为0.30,T等位基因频率为0.70,两组基因型和等位基因频率有显著性差异(P<0.05)。结论ITP患者IL-4启动子-590C→T基因型和等位基因频率存在多态性;IL-4启动子-590C→T多态性可能导致ITP患者血清IL-4及PAIgG升高,在ITP的发病中起一定作用。
Objective To explore the possible relationship between -590C → T polymorphism of human interleukin-4 (IL-4) promoter and idiopathic thrombocytopenic purpura (ITP). Methods Serum IgG and IL-4 levels were detected by enzyme-linked immunosorbent assay (ELISA), and 94 ITP patients and 106 normal healthy volunteers were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) -4 promoter-590C → T genotypes and allele frequencies. Results Serum IL-4 level [(92.43 ± 20.69) ng / L] in ITP group was significantly higher than that in the normal group [(273.71 ± 90.45) ng / 107PA] (46.71 ± 11.9) ng / L]. In ITP group, the frequency of C allele was 0.16 and the T allele frequency was 0.84 at -590 locus in IL-4 promoter region. In normal control group C The allele frequency was 0.30 and the T allele frequency was 0.70. There was significant difference between the two genotypes and allele frequencies (P <0.05). CONCLUSIONS: There is a polymorphism in the -590C → T genotype and allele frequency of IL-4 promoter in ITP patients. IL-4 promoter -590C → T polymorphism may lead to elevated IL-4 and PAIgG levels in ITP patients. ITP play a role in the pathogenesis.