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采用人工接种方法,将烟草立枯病病菌菌株TRs-5接种于烟草云烟87的不同部位,观察其发病效果。研究了菌株接种体在不同接种温度和培养时间对发病效果的影响,并比较了其与稻纹枯病病菌菌株RRs08-22-2接种于水稻品种中优679的致病效果。结果表明,菌株TRs-5既能侵染烟草幼苗近地表的茎基部,导致其发病,也能侵染烟草成株苗叶面形成坏死病斑。在温度28℃环境下接种比在20℃的导致发病更严重。用培养2 d的菌丝体接种比培养4 d的导致更大的病斑。在寄主烟草上,菌株TRs-5比菌株RRs08-22-2的致病力强,但在寄主水稻上,菌株TRs-5比菌株RRs08-22-2的致病力弱。初步发现这2个病菌与2个寄主之间存在致病专化现象。
Using artificial inoculation method, the strain of tobacco leaf blight TRs-5 was inoculated into different parts of tobacco cloud tobacco 87 to observe its onset effect. The effect of inoculum on pathogenicity was studied at different inoculation temperature and culture time, and the pathogenicity of inoculation with rice sheath blight disease strain RRs08-22-2 on Zhongyou 679 was compared. The results showed that the strain TRs-5 could both infect the stem base near the surface of tobacco seedlings and lead to its occurrence. It could also infect the leaf surface of tobacco plants to form necrotic spots. Inoculation at a temperature of 28 ° C is more severe than at 20 ° C. Inoculation with mycelia cultured for 2 days resulted in larger lesions than those cultured for 4 days. On host tobacco, strain TRs-5 was more virulent than strain RRs08-22-2, but on host rice, strain TRs-5 was less virulent than strain RRs08-22-2. Preliminary discovery of these two pathogens and two hosts exist between the disease-specific phenomenon.