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在采用耳蜗顶部灌流方法测定琥珀酸脱氢酶的活性时,本文提出了两点改进,实验动物为豚鼠:(1)孵育前用2%戊二醛磷酸盐缓冲液(pH7.4,4℃)将耳蜗预固定30min,再冰凉(—20——40℃)约3min,以便消除血管纹屏障,使该部位和柯蒂氏器官的琥珀酸脱氢酶活性能同时被显示出来。(2)在孵育液中加入细胞呼吸抑制剂(阿密妥),可使耳蜗背景色变淡,从而减少假阳性现象。通过对一些豚鼠的实验观察,所得结果基本一致。
In the determination of succinate dehydrogenase activity by cochlear top perfusion method, two improvements were proposed in this paper. The experimental animals were guinea pigs: (1) before incubation with 2% glutaraldehyde phosphate buffer (pH 7.4, ) Cochlear pre-fixation 30min, then cold (-20--40 ℃) for about 3min, in order to eliminate the vascular barrier, so that the site and the Keti’s organ succinate dehydrogenase activity can be displayed at the same time. (2) adding cellular respiration inhibitor (Amitosu) into the incubation solution can make the background color of the cochlea fade, thus reducing the false positive phenomenon. Through the experimental observation of some guinea pigs, the results are basically the same.