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目的:对灯盏细辛注射液治疗慢性肾功能不全的作用做动态观察,探求该药改善肾功能的药理机制。方法:健康成年SD大鼠进行5/6肾切除,除假手术组外,选用符合慢性肾功能不全氮质血症条件动物60只,随机分为模型组、尿毒清组、灯盏细辛大剂量组、灯盏细辛中剂量组及灯盏细辛小剂量组,每组12只。于4周、8周和12周动态观察血尿素氮和血肌酐水平,12周时采用ELISA法测定肾组织TNF-α含量,分光光度法进行SOD定量测定,采用SAS8.2e软件进行统计学处理。结果:灯盏细辛注射液各剂量组均有改善肾功能不全的作用,以大剂量组最明显,呈现出量-效关系,但作用弱于尿毒清(P<0.05);灯盏细辛治疗使TNF-α下降和SOD含量升高(P<0.05)。结论:灯盏细辛注射液可改善慢性肾功能不全,其机制可能是通过降低TNF-α水平、促进自由基清除剂SOD的合成。
Objective: To observe the effect of Erigeron breviscapus on chronic renal insufficiency and to explore the pharmacological mechanism of the drug in improving renal function. METHODS: Healthy adult SD rats were subjected to 5/6 nephrectomy. In addition to the sham operation group, 60 animals that met the conditions of chronic renal insufficiency and azotemia were randomly divided into model group, uremic sedative group, and high dose of Erigeron breviscapus. Group, Erigeron breviscapus medium dose group and Erigeron breviscapus low-dose group, 12 in each group. Blood urea nitrogen and serum creatinine levels were dynamically observed at weeks 4, 8 and 12; TNF-α levels were measured by ELISA at 12 weeks; SOD was measured by spectrophotometry; SAS8.2e software was used for statistical analysis. . RESULTS: All doses of Erigeron breviscapus had improvement of renal insufficiency, with the most obvious in large-dose group, showing a dose-effect relationship, but the effect was weaker than that of uremic muscarinone (P<0.05); Erigeron breviscapus treatment TNF-α decreased and SOD increased (P<0.05). Conclusion: Erigeron breviscapus injection can improve chronic renal insufficiency. The mechanism may be through reducing the level of TNF-α and promoting the synthesis of free radical scavenger SOD.