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目的了解高密市食品中小肠结肠炎耶尔森菌及动物带菌情况。方法 2006-2010年采集家畜家禽粪便、冷冻食品共2 097份,4℃冷增菌后用选择性培养基进行病原菌分离培养并作生化鉴定,将可疑菌株做进一步的血清学鉴定和生物分型及毒力检测。结果 2 097份标本中分离出131株小肠结肠炎耶尔森菌,总的检出率为6.25%,其中O∶3(携带毒力基因ail+、ystA+、rbfc+)和O∶9血清型的检出率分别为0.76%和1.52%。108株仅携带ystB基因(82.44%);26株不携带任何基因(19.85%)。131株小肠结肠炎耶尔森菌分布在3个生物型,其中生物1A型占74.80%;生物3型占1.52%;生物4型占0.76%。结论本地区存在致病性小肠结肠炎耶尔森菌,有感染人和动物及引起食源性型疾病的可能,因此必须加大该菌的监测力度,以预防小肠结肠炎耶尔森菌病的感染流行。
Objective To understand the Yersinia enterocolitica and animal carriage in food in Gaomi. Methods A total of 2 097 samples of livestock poultry and frozen food were collected from 2006 to 2010. After cold storage at 4 ℃, selective culture medium was used to isolate and culture pathogenic bacteria for biochemical identification. The suspicious strains were further identified by serology and bio-typing And virulence testing. Results A total of 131 strains of Yersinia enterocolitica were isolated from 2 097 specimens, with a total detection rate of 6.25%. O / 3 (carrying ail +, ystA +, rbfc +) and O: 9 serotypes The rates were 0.76% and 1.52% respectively. 108 strains carried only ystB gene (82.44%); 26 strains did not carry any gene (19.85%). Among 131 strains, Yersinia enterocolitica distributed in 3 biotypes, of which biotype 1A accounted for 74.80%, biotype 3 accounted for 1.52% and biotype 4 accounted for 0.76%. Conclusion Yersinia enterocolitica is present in this area, which may infect humans and animals and cause food-borne diseases. Therefore, it is necessary to increase the monitoring of this bacterium to prevent Yersinia enterocolitica The prevalence of infection.