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目的:探讨毛冬青总黄酮对大鼠局灶性脑缺血-再灌注损伤模型的影响。方法:SD大鼠均分为6组,即假手术、模型、金纳多(40mg/kg)、毛冬青总黄酮组(200mg/kg、100mg/kg、50mg/kg),连续给药5天,模型组及毛冬青总黄酮、金纳多组线栓法制备SD大鼠大脑中动脉栓塞模型,缺血2h,再灌注22h,用Zea Longa法进行神经功能评分,放免法检测血清肿瘤坏死因子(TNF-α)、白细胞介素1β(IL-1β)含量;断头取脑,匀浆,用于超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-Px)、丙二醛(MDA)含量的测定;免疫组化观察海马区促进神经营养因子(BDNF)及血管内皮生长因子(VEGF)蛋白的表达和脑组织形态学的病理变化。结果:与模型组比,毛冬青总黄酮组(200mg/kg、100mg/kg、50mg/kg)能极显著减轻大鼠神经功能缺损评分,降低TNF-α、IL-1β、MDA含量,极显著升高SOD及SGH-PX含量,BDNF及VEGF蛋白的表达,改善大鼠脑组织皮质及海马区的病理损伤情况。结论:毛冬青总黄酮(200mg/kg、100mg/kg、50mg/kg)对大鼠局灶性脑缺血-再灌注损伤模型具有显著的保护作用,其作用机制可能与抑制脂质过氧化、促进神经元的恢复及抑制炎症因子有关。
Objective: To investigate the effect of Mao Dongqing total flavone on focal cerebral ischemia-reperfusion injury in rats. Methods: SD rats were divided into 6 groups randomly: sham operation, model, Ginaton (40mg / kg), Mao Dongqing total flavonoids group (200mg / kg, 100mg / kg, 50mg / kg) , Model group and Mao Dongqing total flavonoids, Ginaton group thread method of middle cerebral artery occlusion model of SD rats, ischemia 2h, reperfusion 22h, using the Zea Longa method for neurological function, radioimmunoassay to detect serum tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) levels were determined. Brain and homogenate were decapitated for superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) (MDA) in the hippocampus. The expression of BDNF and VEGF in the hippocampus was observed by immunohistochemistry and the histopathological changes of the brain were observed. Results: Compared with the model group, total flavonoids of Mao dongqing group (200mg / kg, 100mg / kg, 50mg / kg) could significantly reduce the score of neurological deficit and decrease the content of TNF-α, IL- Increase the content of SOD and SGH-PX, the expression of BDNF and VEGF protein, and improve the pathological damage of cortex and hippocampus in rat brain tissue. Conclusion: Total flavonoids of Mao Dongqing (200mg / kg, 100mg / kg, 50mg / kg) have a significant protective effect on focal cerebral ischemia - reperfusion injury model in rats. The mechanism may be related to inhibition of lipid peroxidation, Promote the recovery of neurons and inhibit the inflammatory cytokines.