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用高胆固醇喂饲+免疫损伤方法建立家兔动脉粥样硬化(AS)模型。应用免疫组织化学染色和原位杂交方法,观察中药穿心莲成分API_(0134)(API)对家兔主动脉壁血小板源生长因子B链蛋白(PDGF-B)、c-sis基因和c-myc基因表达的影响。结果发现:与AS模型组比较,API预防用药能够显著减少主动脉脂质斑块面积百分比(P<0.01)。AS模型组,呈粥样硬化改变的主动脉内膜,可见PDGF-B、c-sis mRNA和c-myc mRNA强阳性表达;API用药组,PDGF-B、c-sis mRNA和c-myc mRNA的表达则被显著抑制。结论:API抑制c-sis和c-myc基因表达的作用,可能是其抗AS的分子机制之一。
A rabbit model of atherosclerosis (AS) was established with high cholesterol feeding and immune injury. Immunohistochemical staining and in situ hybridization were used to observe the platelet-derived growth factor B chain protein (PDGF-B), c-sis gene, and c-myc gene of rabbit aortic wall with andrographis paniculata API_(0134) (API). The effect of expression. The results showed that: compared with the AS model group, API preventive medication can significantly reduce the percentage of aortic lipid plaque area (P <0.01). In the AS model group, atherosclerotic aortic intima was observed, PDGF-B, c-sis mRNA and c-myc mRNA were strongly expressed; API group, PDGF-B, c-sis mRNA and c-myc mRNA were observed. The expression was significantly suppressed. Conclusion: The inhibitory effect of API on c-sis and c-myc gene expression may be one of the molecular mechanisms of anti-AS.