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在泥鳅养殖水体中添加稻秆粉模拟水稻残遗物生境,研究了泥鳅生长和肝胰脏抗氧化酶(SOD、CAT)与解毒酶(GST)活性对转Cry1Ab/Ac基因水稻‘华恢1号’(HH1)的响应。设计以HH1稻秆粉10 mg·L?1、50 mg·L?1、100 mg·L?1和200 mg·L?1 4个梯度浓度处理泥鳅为试验组,以非转Bt基因水稻‘明恢63’(MH63)稻秆粉处理组为阴性对照,不加稻秆粉的基础饲养组为空白对照。结果显示:在4种稻秆粉浓度下,HH1组与MH63对照组泥鳅的特定生长率、肥满度、内脏系数及SOD、CAT和GST酶活性均无显著差异(P>0.05);与空白对照比较,稻秆粉浓度升高对泥鳅生长的抑制逐渐增强,当浓度达到200 mg·L?1时,HH1组和MH63对照组泥鳅的特定生长率、内脏系数与CAT活性降低。研究结果表明,水体中低含量的转融合基因Cry1Ab/Ac水稻HH1稻秆粉对泥鳅的生长与生理酶活性没有明显影响,高浓度HH1和MH63稻秆粉均使泥鳅的生长和生理酶活性显著降低,这可能与养殖水体中浓度较高的悬浮稻秆粉妨碍了泥鳅的呼吸和滤食,及稻秆粉的分解降低了水体p H和溶氧量有关。
The effects of SOD, CAT and detoxification enzyme (GST) activity on the growth of loach and the effects of the transgenic Cry1Ab / Ac transgenic rice ’Hua Hui No.1’ ’(HH1) response. The experiment was conducted to treat loach with the concentration of HH1 rice straw 10 mg · L -1, 50 mg · L -1, 100 mg · L -1 and 200 mg · L -1, and the non-transgenic Bt rice ’ Minghui 63 ’(MH63) rice straw powder treatment group as a negative control, without the basic group of rice straw powder as a blank control. The results showed that there was no significant difference in specific growth rate, fatness, visceral index and activities of SOD, CAT and GST among the HH1 and MH63 control groups at the four rice straw concentrations (P> 0.05) Compared with the control, the inhibition of growth of loach increased with increasing rice straw concentration. When the concentration reached 200 mg · L -1, the specific growth rate, visceral index and CAT activity of loach decreased in HH1 and MH63 control groups. The results showed that the low content of fusion gene Cry1Ab / Ac rice HH1 rice straw powder had no significant effect on the growth and physiological enzyme activity of loach. High concentrations of HH1 and MH63 rice straw powder all induced the growth and physiological enzyme activities of loach Decreased, which may be related to the concentration of suspended rice straw in aquaculture water impede the respiratory and filter loach feeding, and the decomposition of rice straw powder reduces water p H and dissolved oxygen related.