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目的:研究成纤维细胞激活蛋白((fibroblast activation protein alpha,FAPA)mRNA在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)及配对正常口腔黏膜组织中的表达。方法:采用RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常口腔黏膜中FAPAmRNA的表达;所得数据用SAS6.12软件包进行χ2检验和t检验。结果:RT-PCR检测结果表明,FAPAmRNA在30例OSCC和配对正常黏膜的阳性率分别为83%(25/30)和40%(12/30)(P<0.001);总表达水平分别为3.58±0.46和1.27±0.21,上调2.82倍(P<0.0001)。结论:在OSCC的癌变过程中,FAPA基因过表达,可能是OSCC的一个靶基因;FAPA在OSCC诊断中具有应用价值。
Objective: To study the expression of fibroblast activation protein alpha (FAPA) mRNA in oral squamous cell carcinoma (OSCC) and matched normal oral mucosa.Methods: The expression of FAPA mRNA was detected by RT-PCR The expression of FAPAmRNA in 30 cases of OSCC and its normal oral mucosa tissues was analyzed by SPSS 11.5 software package.Results: RT-PCR results showed that FAPA mRNA was positive in 30 cases of OSCC and matched normal The positive rates of mucosa were 83% (25/30) and 40% (12/30) respectively (P <0.001), and the total expression levels were 3.58 ± 0.46 and 1.27 ± 0.21 respectively, up by 2.82 times (P <0.0001) .Conclusion : Overexpression of FAPA gene during the carcinogenesis of OSCC may be a target gene of OSCC. FAPA is of value in the diagnosis of OSCC.