论文部分内容阅读
SPF级6周龄Balb/c雄性小鼠60只,随机分为3组,每组20只,适应饲养1周后进行动物试验。雌激素+Gh-relin试验组:前2周隔日腹腔注射苯甲酸雌二醇注射液(含苯甲酸雌二醇0.1mg)0.05mL/只,后2周按每日腹腔注射Ghrelin(含Ghrelin 60μg)0.1mL/只;雌激素+生理盐水对照组:前2周隔日腹腔注射苯甲酸雌二醇注射液(含苯甲酸雌二醇0.1mg)0.05mL/只,后2周按每日腹腔注射0.9%生理盐水0.1mL/只;生理盐水对照组:前2周按0.05mL/只隔日腹腔注射0.9%生理盐水,后2周按0.1mL/只每日腹腔注射0.9%生理盐水。动物试验结束后,统计小鼠胸腺指数,应用光镜观察胸腺形态学变化,采用实时荧光定量PCR法检测胸腺中12种细胞因子mRNA表达量的变化。结果显示,注射Ghrelin后,雌二醇诱导的小鼠胸腺萎缩在形态学上基本恢复到正常水平,胸腺中白细胞介素1(IL-1)、IL-2、IL-4、IL-6、IL-12、干扰素(IFN-γ)、白血病抑制因子(LIF)、抑瘤素(OSM)、干细胞因子(SCF)、胸腺体液因子(THF)、肿瘤坏死因子(TNF-α)mRNA含量显著降低(P<0.05),而IL-7mRNA含量稍微升高(P>0.05)。结果表明,Ghrelin对雌激素诱导的小鼠胸腺萎缩具有逆转作用,其机制可能是:一方面通过抑制IL-6、OSM、LIF、SCF等细胞因子的表达,从而促进胸腺细胞的增殖;另一方面通过抑制IL-1、IL-2、IL-4、IL-12、THF等细胞因子的表达,从而减少TNF-α和IFN-γ的分泌,进而抑制胸腺细胞的凋亡。
Sixty Balb / c male mice of SPF grade were randomly divided into three groups with 20 animals in each group. Estrogen + Ghrelin test group: 2 weeks before the intraperitoneal injection every other day injections of estradiol benzoate (containing estradiol benzoate 0.1mg) 0.05mL / only after two weeks by daily intraperitoneal injection of Ghrelin (containing Ghrelin 60μg ) 0.1mL / only; estrogen + saline control group: the first 2 weeks after the intraperitoneal injection of estradiol benzoate injection (containing 0.1mg benzoate) 0.05mL / only 2 weeks after the daily intraperitoneal injection 0.9% saline 0.1mL / only; saline control group: 0.9mL saline was injected intraperitoneally at a rate of 0.05mL / every other two weeks and 0.9mL saline at 0.1mL per daily for 2 weeks. After the end of the animal experiment, the thymus index of the mice was counted, the morphology of the thymus was observed with light microscope, and the changes of mRNA expression of 12 cytokines in thymus were detected by real-time fluorescence quantitative PCR. The results showed that, after injection of Ghrelin, thymic atrophy induced by estradiol in morphology mice recovered to normal levels, thymus interleukin 1 (IL-1), IL-2, IL-4, IL-6, IL-12, interferon (IFN-γ), leukemia inhibitory factor (LIF), oncostatin (OSM), stem cell factor (SCF), thymic humoral factor (THF), mRNA content of tumor necrosis factor (TNF-α) was significantly (P <0.05), while the level of IL-7 mRNA increased slightly (P> 0.05). The results showed that Ghrelin could reverse the estrogen-induced thymus atrophy in mice. The mechanism may be that: on the one hand, thymocyte proliferation can be promoted by inhibiting the expression of cytokines such as IL-6, OSM, LIF and SCF; By inhibiting the expression of cytokines such as IL-1, IL-2, IL-4, IL-12 and THF, the secretion of TNF-α and IFN-γ can be decreased and the apoptosis of thymus cells can be inhibited.