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目的:探讨孕妇不规则抗体检测在非ABO新生儿溶血病(HDN)中的意义。方法:用试管法、微柱凝胶法、抗人球蛋白法、盐水法对2 772例孕妇进行血型不规则抗体筛选和鉴定,阳性者进一步检测其抗体特异性,抗体效价测定和免疫球蛋白类型检定。结果:2 772例孕妇中检测25例不规则抗体,其中抗D 8例,抗E 3例,抗D+C 2例,抗A+抗Ec 1例,抗M 4例,抗Mur合并抗C 1例,抗Lea 1例,抗LebH 1例,抗HI 1例,其他未检出抗体3例。10例新生儿被来自母体的不规则抗体所致敏,出现不同程度HDN。结论:孕妇不规则抗体的筛选、鉴定、性质类型和抗体效价等检测都有助于非ABO-HDN的早期诊断,评估HDN的严重程度,从而有效的预防并及时治疗核黄疸,水肿等严重并发症有较大意义。
Objective: To investigate the significance of detecting irregular antibody in pregnant women in non-ABO neonatal hemolytic disease (HDN). Methods: A total of 2 772 pregnant women were screened and identified for blood type irregular antibody by means of in vitro test, microcolumn gel method, anti-human globulin method and saline method. Positive samples were further tested for antibody specificity, antibody titer and immunoglobulin Protein type test. Results: There were 25 irregular antibodies in 2 772 pregnant women, including 8 cases against D, 3 cases against E, 2 cases against D + C, 1 case against A + anti-Ec, 4 cases against M, For example, 1 case was anti-Lea, 1 case was anti-LebH, 1 case was anti-HI, and 3 cases did not detect other antibodies. Ten newborns were sensitized by irregular antibodies from the mother, with varying degrees of HDN. Conclusion: The detection of irregular antibodies in pregnant women can be helpful to the early diagnosis of non-ABO-HDN and to evaluate the severity of HDN so as to effectively prevent and treat severe cases of nuclear jaundice and edema Complications have great significance.