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诱发突变是作物改良的一种重要手段。作者用Υ射线和甲基磺酸乙酯(EMS)对两个绿豆品种“ML-5”和“K-851“进行单一处理和复合处理,以诱发绿豆的有用突变。(1)对绿豆种子进行20kR、30kR、40kR和50kR的钴-60Υ射线辐照处理;(2)绿豆种子先在水中浸2小时,然后用EMS水溶液(0.2％和0.4％的浓度)处理6和12小时,处理完毕,用水洗涤种子,并把种子放在吸水纸上吸干水分;(3)对经20、30、40和50kRΥ射线辐照处理的种子,再用02％的EMS水溶液浸6小时,后处理同于EMS单一处理。各处理种子均为300颗,重复三次,随机区组设计种植成M_1代,成熟时单株收获种子。M_2代按株行法
Mutagenesis is an important tool for crop improvement. The authors used a combination of γ-rays and EMS to make a single treatment and compound treatment of two mung bean varieties ”ML-5“ and ”K-851" to induce useful mutations in mung bean. (1) The mung bean seeds were irradiated with 20 kR, 30 kR, 40 kR and 50 kR cobalt-60 γ rays; (2) the mung bean seeds were immersed in water for 2 hours and then treated with aqueous EMS solution (0.2% and 0.4% And 12 hours. After the treatment, the seeds were washed with water and the seeds were blotted on absorbent paper to dry the water. (3) Seeds irradiated with 20, 30, 40 and 50 kRγ rays were further immersed in 02% aqueous EMS solution 6 hours, the same treatment with the EMS single treatment. Each seed was treated 300 times, repeated three times, randomized block design planted into M_1 generation, harvesting seeds at maturity. M_2 generation line by law