1989年我们在安国县舍二村对1986年检出人毛滴虫的13户家庭50人和该村幼儿园19名幼儿,共收集69份新鲜粪便,同时应用4种方法进行原虫检查,结果报告如下: 1 试管滤纸培养法 基本与全国寄生虫分布调查所用方法相同,不同点是试验所用物品均经高压灭菌。标本接种后置25—30℃培养3—4d,取出滤纸条,继续培养到第7d。滤纸条取出时不用温水浸洗纸条,避免混浊的培养液影响原虫滋养体检出。 In 1989, we found 21 families of Trichomonas and 13 young people of 19 nurseries in 1986 in Chenshe Village, Anguo County. A total of 69 fresh stools were collected and four kinds of methods were used for protozoa examination. The results are reported as follows : 1 tube filter paper culture method basically the same as the survey of the distribution of parasites in the country, the difference is that the test items are autoclaved. Specimens were incubated at 25-30 ℃ for 3-4 days after inoculation, and the filter paper was removed and cultured until the 7th day. Remove the filter paper without warm dip paper, to avoid the turbid culture solution of protozoan trophozoite detected.