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利用精子稀释液(0.5%氯化钠+0.25%氯化钾+0.25%葡萄糖)分别配制4%、8%、12%、16%、20%、24%的二甲亚砜(DMSO)、甘油(Glycerol)、1,2-丙二醇(1,2-Propanediol)、乙二醇(Ethyleneglycol)四种抗冻剂,对日本鳗鲡精子进行激活处理,观察记录精子在不同浓度抗冻剂中的活力和5种运动(激烈运动、快速运动、慢速运动、摆动、死亡)时间。结果表明:4%~24%的四种抗冻剂都可以激活日本鳗鲡精子,其中二甲亚砜浓度为12%时,精子活力最高,平均可达到78.3%;甘油浓度为8%时,精子活力最高,平均可达到72.8%;12%的1,2-丙二醇和乙二醇激活精子后,精子活力都可以达到最高,分别是47.6%和57.8%。日本鳗鲡精子在8%的二甲亚砜、8%的甘油、8%的1,2-丙二醇和8%的乙二醇中,激烈运动时间均为最长,分别为11.8 s、11.1 s、6.0 s和2.9 s;用8%的甘油、16%的二甲亚砜、16%的1,2-丙二醇和12%的乙二醇激活日本鳗鲡精子后,精子存活时间均最长,分别达到154.6 s、45 s、20 s和34 s。与其它几种抗冻剂相比,8%的甘油能显著延长日本鳗鲡精子的寿命。四种抗冻剂对日本鳗鲡精子都有一定的毒性作用,处理后精子的活力和寿命均降低。
4%, 8%, 12%, 16%, 20%, 24% dimethylsulfoxide (DMSO) and glycerol were separately prepared with sperm diluent (0.5% sodium chloride + 0.25% potassium chloride + 0.25% glucose) Glycerol, 1,2-Propanediol and Ethyleneglycol antifreeze were used to activate sperm in Japanese eel, and the activity of sperm in different concentrations of antifreeze and Five kinds of exercise (intense exercise, rapid exercise, slow motion, swing, death) time. The results showed that 4% ~ 24% of four cryoprotectants could activate Japanese eel sperm, and the highest sperm motility was 78.3% at dimethylsulfoxide concentration of 12%. When glycerol concentration was 8%, sperm The highest activity, with an average of 72.8%; sperm motility can reach the highest after 12% 1,2-propanediol and ethylene glycol activated sperm, respectively 47.6% and 57.8%. Japanese eel sperm had the longest exercise time of 8% dimethylsulfoxide, 8% glycerol, 8% 1,2-propanediol and 8% ethylene glycol, respectively, for 11.8 s, 11.1 s, 6.0 s and 2.9 s. The sperm survival time was the longest with 8% glycerol, 16% dimethylsulfoxide, 16% 1,2-propanediol and 12% ethylene glycol, respectively 154.6 s, 45 s, 20 s and 34 s. Compared with several other cryoprotectants, 8% glycerol can significantly prolong the life of Japanese eel sperm. Four kinds of anti-freezing agent on Japanese eel sperm have a certain degree of toxicity, after treatment of sperm vitality and life expectancy are reduced.