腹腔注射Q热立克次体悬液感染BALB/c小鼠,发病后解剖,取主要组织脏器,应用免疫组化和原位杂交检测感染小鼠体内Q热立克次体的抗原分布和DNA在靶细胞中的表达,探讨Q热病变规律及致病机理。结果显示阳性信号多位于单核-巨噬细胞系统细胞胞浆内。结果提示免疫组化和原位杂交技术可以作为Q热特异性的诊断方法,并为致病机理的研究提供线索。 Infected BALB / c mice were inoculated intraperitoneally with Q rickettsia gondii. After the onset of disease, the tissues were dissected and the tissues of major organs were harvested. The antigenic distribution of T. rickettsi in infected mice was detected by immunohistochemistry and in situ hybridization. DNA expression in target cells to explore the Q fever pathology and pathogenesis. The results showed that the positive signals were mostly located in the cytoplasm of monocyte-macrophage system. The results suggest that immunohistochemistry and in situ hybridization can be used as a diagnostic method of Q-heat specificity and provide clues for the study of pathogenic mechanism.