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目的:探讨三七总皂苷(total panax notoginseng saponins,tPNS)对脊髓半横断损伤后对脑源性神经营养因子(Brain-derivedneurotrophic factor,BDNF)表达以及运动功能恢复的作用的影响。方法:大鼠随机分为正常组和实验组,实验组大鼠脊髓T10右侧半横断模型,损伤后15min,腹腔注射三七总皂苷,剂量为20mg.kg-1,以后每天给药一次,溶媒对照组注射等量生理盐水。术后进行BBB评分和斜板实验检测;动物分别存活1d、3d、7d、14d、28d后,采用免疫荧光化学方法检测脊髓损伤远侧端BDNF表达的变化。结果:BBB评分及斜板实验结果显示,三七总皂苷能明显促进脊髓损伤后运动功能的恢复,尤其是损伤后7d和14d,三七总皂苷组评分明显高于溶媒对照组。免疫组化结果显示:脊髓半横断损伤后,损伤远侧端损伤侧BDNF的表达强于对侧,损伤侧BDNF的表达呈现出1d,3d逐渐增强,7d达高峰的趋势,14dBDNF的表达逐渐下降,至28d仍略高于正常组。三七总皂苷组和溶媒对照组相比,BDNF表达的时间趋势相同,但相同时间点BDNF的表达强于对照组,尤其是3d、7d。结论:三七总皂苷能增强脊髓半横断损伤后BDNF的表达,这可能是其改善脊髓再生的微环境,促进脊髓损伤后运动功能恢复的机制之一。
Objective: To investigate the effect of total panax notoginseng saponins (tPNS) on the expression of brain-derived neurotrophic factor (BDNF) and the recovery of motor function after spinal cord hemisection. Methods: The rats were randomly divided into normal group and experimental group. T10 right hemisected model of spinal cord in experimental group was injected intraperitoneally with Panax notoginseng saponins at a dosage of 20 mg.kg-1, The vehicle control group was injected with the same amount of saline. BBB score and oblique plate test were performed postoperatively. After the animals survived for 1, 3, 7, 14 and 28 days respectively, the expression of BDNF on the distal side of spinal cord injury was detected by immunofluorescence staining. Results: The results of BBB score and inclined plate showed that Panax notoginseng Saponins could obviously promote the recovery of motor function after spinal cord injury, especially at 7d and 14d after injury, the score of Panax notoginseng saponin group was significantly higher than that of vehicle control group. The results of immunohistochemistry showed that the expression of BDNF on the injured side was stronger than that on the contralateral side, and the expression of BDNF was gradually increased on the 1d and 3d and reached the peak on the 7d, and the expression of 14dBDNF was gradually decreased , Still slightly higher than the normal group till 28d. Compared with the vehicle control group, the time trend of BDNF expression in Panax notoginseng saponins group was the same, but the expression of BDNF in the same time point was stronger than that in the control group, especially 3d and 7d. Conclusion: Panax notoginseng saponins can enhance the expression of BDNF after spinal cord hemi-transection, which may be one of the mechanisms that improve the microenvironment of spinal cord regeneration and promote motor function recovery after spinal cord injury.