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为了解育成抗病品种抗性稳定性及胞囊线虫生理小种侵染力的变异,于2005 ～2008年,应用黑龙江省发生的大豆胞囊线虫3号生理小种及已经通过鉴定的抗大豆胞囊线虫3号生理小种的品种,哈尔滨小黑豆、灰皮支等8个品种和感病对照品种Lee68为材料,进行抗病基因对大豆胞囊线虫3号生理小种选择作用研究。在盆栽条件下,强迫大豆胞囊线虫3号生理小种的群体在抗病品种上繁殖10代,选用5个标准鉴定大豆胞囊线虫生理小种的鉴别品种,重新鉴定经抗病基因选择后的大豆胞囊线虫的生理小种类型。结果表明:原来为3号生理小种的线虫群体,经在抗病品种抗线虫1号、抗线虫2号、抗线虫3号、抗线虫4号、抗线虫5号上连续选择10代之后,变为6号小种;经在灰皮支黑豆上选择之后变为10号小种;经在Peking上选择之后变为14号小种,经在哈尔滨小黑豆上选择之后变为15号小种。上述鉴定结果说明,原大豆胞囊线虫生理种群体,经过在抗病品种上连续强迫繁殖后,形成新的生理小种,并使原抗病品种变为感染品种,认为在生产上采用轮作方式是保持抗线品种抗性稳定性的有效途径。
In order to understand the resistance stability of resistant varieties and the variation of invasive ability of cyst nematode physiological races, the physiological characteristics of soybean cyst nematode No.3 and Heilongjiang Province, which have passed the identification of anti-soybean Eight species of cyst nematode No.3 physiological races, small black beans and gray peel of Harbin, and Lee68, a susceptible cultivar, were selected to study the effects of disease resistance genes on the selection of the race of soybean cyst nematode No.3. Under potted condition, the population of Soybean Cyst Nematode No. 3 was inoculated on the resistant varieties for 10 passages. Five discriminant varieties of Soybean cyst nematode physiological races were selected and identified again after resistance gene selection Soybean cyst nematode physiologic races. The results showed that the nematode population which originally belonged to physiological race No. 3 was continuously selected for 10 generations against the resistant varieties Nematode 1, Nematode 2, Nematode 3, Nematode 4 and Nematode 5, Becomes racier No. 6; becomes raccoon 10 after being selected on the petiole of black peel; becomes raccoon 14 after being selected on Peking, becomes rauric 15 after being selected on the mulberry black in Harbin . The identification results show that the original soybean cyst nematode physiological population, after continuous forced breeding on the resistant varieties, the formation of new physiological races, and the original disease-resistant varieties into infected varieties, that the production of rotation mode It is an effective way to maintain the resistance stability of resistant lines.