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目的 观察雷公藤多甙（ＧＴＷ）对体外培养成骨细胞（ＯＢ）增殖的影响，以进一步探讨雷公藤（ＴＷ）致女性骨质疏松的机制。方法 以ＳＤ大鼠ＯＢ增殖为模型，分离新生ＳＤ大鼠头盖骨ＯＢ，传代后加入不同浓度ＧＴＷ，用ＭＴＴ比色分析法检测ＧＴＷ在体外对ＯＢ增殖的作用和效价。结果 培养２ｄ，ＧＴＷ１μｇ／ｍｌ起显著抑制ＯＢ增殖（Ｐ＜０．０１）；培养４ｄ，ＧＴＷ０．１μｇ／ｍｌ起显著抑制ＯＢ增殖（Ｐ＜０．０５）。药物对ＯＢ增殖的抑制程度与其剂量呈正相关（Ｐ＜０．０５）。结论 ＧＴＷ显著抑制ＯＢ增殖且呈剂量依赖性。ＴＷ对ＯＢ的直接抑制作用，是其导致药物性骨质疏松的一个重要原因。
Objective To observe the effect of tripterygium wilfordii glycosides (GTW) on the proliferation of cultured osteoblasts (OB) in vitro and to explore the mechanism of female osteoporosis caused by TW. Methods The OB of SD rats was isolated from adult OB rat models. After passage, different concentration of GTW was added. The effect of GTW on OB proliferation was evaluated by MTT colorimetric assay. Results After cultured for 2 days, GTW1μg / ml significantly inhibited OB proliferation (P <0.01). After cultured for 4 days, GTW 0.1μg / ml significantly inhibited OB proliferation (P <0.05). The inhibitory effect of drugs on OB proliferation was positively correlated with its dosage (P <0.05). Conclusion GTW significantly inhibits OB proliferation in a dose-dependent manner. The direct effect of TW on the inhibition of OB is an important reason for the drug-induced osteoporosis.