用四个盖玻片及两个培养瓶对每24ml羊水进行培养。数目畸变得自盖玻片培养,从10个或更多的集落平均计数15个细胞,可在9～14天内得出结果。培养瓶培养在12～16天收获,可得质量好的前中期染色体,对诊断染色体的结构畸变特别有用。用Eagle基本培养液,20%胎牛血清,补充1.2mML—谷氨酰胺,0.9%mM丙酮酸,青霉素(100IU/ml)和链霉素(100μg/ml),pH7.2～7.4。 Each 24 ml of amniotic fluid was cultured with four coverslips and two flasks. The number of aberrations was obtained from a cover slip culture and an average of 15 cells from 10 or more colonies yielded results in 9 to 14 days. Culture flasks are harvested from 12 to 16 days to obtain good-quality, pre-metaphase chromosomes that are particularly useful for diagnosing structural aberrations in the chromosome. Eagle’s basic medium, 20% fetal bovine serum, supplemented with 1.2 mM L-glutamine, 0.9% mM pyruvate, penicillin (100 IU / ml) and streptomycin (100 g / ml), pH 7.2 to 7.4.