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目的:分析酸模、皱叶酸模、巴天酸模、长刺酸模、毛脉酸模、羊蹄酸模的psbA-trnH条形码序列,探讨酸模属植物的鉴定新方法。方法:对24份植物样品提取基因组DNA,PCR聚合酶链式反应扩增psbA-trnH序列进行双向测序,序列经过Condon Code Aligner拼接后,采用MEGA 6.0软件进行序列比对,计算种内种间K2P遗传距离,构建系统邻接树(NJ Tree)。结果:表明酸模属psbA-trnH序列种内平均K2P距离均小于种间平均K2P距离;应用相似性搜索法分析结果表明psbA-trnH序列可准确鉴定酸模属植物;NJ树显示除巴天酸模和皱叶酸模聚为一支,其余酸模属植物表现出良好的单系性。结论:psbA-trnH序列可用于鉴定酸模属植物,为酸模属植物的临床安全用药提供依据。
OBJECTIVE: To analyze the psbA-trnH barcode sequence of acid mold, folic acid mold, acid form of Pachytene, long thorn acid mold, furosemide acid mold, and lactis acid mold, and to explore a new method of identification of acid mold plants. Methods: Genomic DNA was extracted from 24 plant samples. The sequence of psbA-trnH was amplified by PCR and sequenced by Condon Code Aligner. The sequences were aligned by MEGA 6.0 software to calculate the intraspecific K2P Genetic distance, construct a system of adjacent tree (NJ Tree). The results showed that the average K2P distances of species psbA-trnH were less than the average distance between species K2P. The results of similarity search showed that psbA-trnH could be used to identify the genus spp. Folic acid mold together, and the rest of the acid mold plants showed a good monophyletic. Conclusion: The psbA-trnH sequence can be used for identification of acid mold plants and provide the basis for the clinical safety of sour plants.