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目的:观察白细胞介素-35(IL-35)在扩张型心肌病(DCM)患者外周血单个核细胞(PBMCs)中表达和血浆水平变化,探讨其在DCM发病中的作用。方法:选择对象为30例DCM的患者(DCM组),24例心力衰竭的患者(HF组)及30例健康体检者为正常对照组。用逆转录-聚合酶链反应(RT-PCR)测定PBMCS的IL-35特异性亚基EB病毒诱导基因3(EBI3)mRNA的表达。用酶联免疫吸附法(ELISA)检测血浆中IL-35水平。结果:①DCM组PBMCs表达IL-35的EBI3mRNA水平(0.15±0.03)明显降低于HF组(0.34±0.08)及正常对照组(0.33±0.07)(均P<0.01),而HF组及正常对照组间差异无统计学意义(P>0.05);②DCM组(24.08±4.40)血浆IL-35的水平明显低于HF组(53.03±5.04)和正常对照组(43.89±8.01)(均P<0.01),而HF组及正常对照组间差异无统计学意义(P>0.05);③DCM组血浆中IL-35含量与射血分数呈正相关(r=0.449,P<0.05),与心功能NYHA分级呈负相关(r=-0.839,P<0.01)。结论:DCM患者的IL-35表达下调,提示IL-35可能在DCM的免疫发病机制中起了一定作用。
Objective: To investigate the expression and plasma level of interleukin-35 (IL-35) in peripheral blood mononuclear cells (PBMCs) of patients with dilated cardiomyopathy (DCM) and to explore its role in the pathogenesis of DCM. Methods: Thirty patients with DCM (DCM group), 24 patients with heart failure (HF group) and 30 healthy controls were selected as normal control group. The IL-35-specific subunit EBV3-induced gene 3 (EBI3) mRNA expression in PBMCs was determined by reverse transcription-polymerase chain reaction (RT-PCR). Plasma IL-35 levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: ① The level of EBI3 mRNA in PBMCs expressing IL-35 in DCM group (0.15 ± 0.03) was significantly lower than that in HF group (0.34 ± 0.08) and normal control group (0.33 ± 0.07) (both P <0.01) There was no significant difference between the two groups (P> 0.05); ② The level of plasma IL-35 in DCM group (24.08 ± 4.40) was significantly lower than that in HF group (53.03 ± 5.04) and normal control group (43.89 ± 8.01) , But there was no significant difference between HF group and normal control group (P> 0.05). ③ The plasma level of IL-35 in DCM group was positively correlated with ejection fraction (r = 0.449, P <0.05) Negative correlation (r = -0.839, P <0.01). Conclusion: Down-regulation of IL-35 expression in DCM patients suggests that IL-35 may play a role in the pathogenesis of DCM.