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本文用RP—HPLC法测定复方土槿皮酊中苯甲酸和水杨酸的含量,在ODS柱上,以0.02mol/L KH_2PO_4—甲醇(90∶10)为流动相,于254nm波长处检测,水杨酸和苯甲酸得到良好分离。该法对样品处理简单,可用于生产过程和成品的质量控制。
The contents of benzoic acid and salicylic acid in compound Smilax glabra were determined by RP-HPLC. The mobile phase was 0.02 mol/L KH 2 PO 4 -methanol (90:10) on ODS column and detected at 254 nm wavelength. Salicylic acid and benzoic acid are well separated. The method is simple to handle the sample and can be used for quality control of the production process and finished product.