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目的:探讨平喘汤对哮喘大鼠模型气道壁厚度及转化生长因子-β1 mRNA(TGF-β1 mRNA)和Ⅲ型胶原表达的影响及可能的作用机制。方法:将40只雄性Wistar大鼠随机分为正常对照组、哮喘组、地塞米松组、平喘汤组,每组10只。用卵白蛋白(OVA)致敏吸入激发制备哮喘模型。在哮喘激发4周后测量肺组织的TGF-β1 mRNA和气道壁Ⅲ型胶原的表达。结果:模型组TGF-β1mRNA、Ⅲ型胶原表达明显高于正常对照组(P<0.05);平喘汤组和地塞米松组TGF-β1 mRNA、Ⅲ型胶原表达显著低于模型组(P<0.05);气道壁厚度模型组明显高于正常对照组(P<0.05);平喘汤组和地塞米松组显著低于模型组(P<0.05)。结论:平喘汤通过降低TGF-β1 mRNA的过度表达,抑制Ⅲ型胶原的沉积,减轻气道重塑的发生。
Objective: To investigate the effect of Pingchuan Decoction on the airway wall thickness and the expression of TGF-β1 mRNA and type Ⅲ collagen in asthmatic rat model and its possible mechanism. Methods: Forty male Wistar rats were randomly divided into normal control group, asthma group, dexamethasone group and Pingchuan Decoction group, with 10 rats in each group. Asthma models were prepared by inhalation challenge with ovalbumin (OVA). After 4 weeks of asthma challenge, the expression of TGF-β1 mRNA and type III collagen in airway wall were measured. Results: The expression of TGF-β1 mRNA and type Ⅲ collagen in model group was significantly higher than that in normal control group (P <0.05). The expression of TGF-β1 mRNA and type Ⅲ collagen in model group was significantly lower than that in model group (P < 0.05). The airway wall thickness in the model group was significantly higher than that in the normal control group (P <0.05). The levels in the asthma group and dexamethasone group were significantly lower than those in the model group (P <0.05). Conclusion: Pingchuan Decoction can reduce the deposition of type Ⅲ collagen and reduce the remodeling of airway by reducing the overexpression of TGF-β1 mRNA.