目的　研究雌激素在体外对骨形成的影响。方法　采用胚胎小鼠长骨培养模型 ,测定不同浓度雌酚酮对长骨钙、磷、羟脯氨酸含量和碱性磷酸酶活性的影响。结果　当培养介质中雌酚酮浓度为 10 7mol/L时 ,培养 48h后 ,长骨钙、磷、羟脯氨酸含量和碱性磷酸酶活性与对照组相比显著升高。结论　提示一定浓度的雌激素在体外可促进骨形成 Objective To study the effect of estrogen on bone formation in vitro. Methods The long-bone culture model of embryonic mouse was used to determine the effects of different concentrations of estrone on calcium, phosphorus, hydroxyproline content and alkaline phosphatase activity in long bone. Results When the concentration of estrone in culture medium was 10 7 mol / L, the contents of calcium, phosphorus, hydroxyproline and alkaline phosphatase in long bone were significantly increased compared with those in control group. Conclusions suggest that a certain concentration of estrogen can promote bone formation in vitro