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目的观察noggin基因能否单独将人骨髓间充质干细胞诱导为神经细胞以成为挽救神经退行性病变的“种子细胞”。方法原代培养人骨髓间充质干细胞,分为对照组、空白载体转染的Ad组和含有noggin基因的腺病毒载体转染的Ad-noggin组,观察转染后48h、4、7、10d等时相点各组细胞的形态学变化并行统计学分析。结果2种载体皆可成功转染人骨髓间充质干细胞使其自发绿色荧光,但Ad组形态无显著变化,而Ad-noggin组细胞在转染后48h即可见少量细胞分化为神经样细胞,转染后4d可见神经样细胞数目增多且伸出少量神经细胞样突起,10d时分化的细胞明显增多。与转染后7d相比,Ad-noggin组转染10d时胞体直经和树突直径明显增加(P<0.05),但形态无改变。结论单独以含noggin基因的腺病毒载体转染人骨髓间充质干细胞可以使其向神经样细胞分化。
Objective To observe whether the noggin gene can induce human bone marrow mesenchymal stem cells to become nerve cells alone to become “seed cells” for rescuing neurodegenerative diseases. Methods Primary human bone marrow mesenchymal stem cells were cultured and divided into control group, blank vector transfected Ad group and Ad-noggin group transfected with adenovirus vector containing noggin gene. Isochronism point of each group of cells morphological changes parallel statistical analysis. Results Both vectors could successfully transfect human bone marrow mesenchymal stem cells to spontaneous green fluorescence, but there was no significant change in the morphology of Ad group. Ad-noggin cells differentiated into neuron-like cells 48h after transfection, Four days after transfection, the number of neuron-like cells increased and a small number of neurocyte-like protuberances protruded, which markedly increased on the 10th day. Compared with the 7th day after transfection, the diameter of straight and dendrites of Ad-noggin group increased significantly (P <0.05) 10d after transfection, but the morphology did not change. Conclusion Human bone marrow mesenchymal stem cells transfected with adenovirus vector containing noggin gene can differentiate into neuron-like cells.