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目的了解普洱市2012年手足口病的病原体情况,为手足口病的防控和治疗提供实验室依据。方法采集本市各级医疗机构临床诊断为手足口病患者的咽拭子和疱疹液标本,提取病毒核酸,采用Real-time RT-PCR法,检测肠道病毒71型(EV71)和柯萨奇病毒A16型(CoxA16)。结果 2012年共检测552份手足口病病人标本,肠道病毒核酸阳性284份,阳性率51.45%,CoxA16阳性率为29.71%(164/552),EV71阳性率为21.74%(120/552)。结论 2012年普洱市手足口病病原以EV71和CoxA16为主,5岁以下儿童是肠道病毒感染的高危人群,儿童肠道病毒感染无明显性别差异。
Objective To understand the pathogens of hand, foot and mouth disease in Pu’er City in 2012 and provide a laboratory basis for the prevention and treatment of hand-foot-mouth disease. Methods Throat swabs and herpes fluid samples of clinically diagnosed hand, foot and mouth disease patients were collected from the medical institutions at different levels of the city. The viral nucleic acids were extracted. Real-time RT-PCR was used to detect EV71 and Coxsackie Virus A16 type (CoxA16). Results A total of 552 HFMD patients were detected in 2012, 284 were positive for enterovirus nucleic acid, the positive rate was 51.45%. The positive rate of CoxA16 was 29.71% (164/552). The positive rate of EV71 was 21.74% (120/552). Conclusion The pathogens of HFMD in Pu’er City in 2012 were mainly EV71 and CoxA16. Children under 5 years of age were at high risk of enterovirus infection. There was no significant gender difference in children with enterovirus infection.